- #Phosphorylated asyn stereology how to#
- #Phosphorylated asyn stereology series#
- #Phosphorylated asyn stereology free#
Thus, one purpose of this book is to dispel any confusion and provide a clear and detailed road map of how to successfully design and execute a gene therapy experiment in order to obtain consistent results. Although such discrepancies are not the result of any wrongdoing, their occurrence adds to the mysticism sometimes associated with gene therapy and could serve to reduce the enthusiasm for taking on similar projects in the future. Therefore, it is all too common that studies involving gene therapy manipulations produce results that vary between investigators (e.g., Ref. Rather, this esoteric, yet essential knowledge is either briefly mentioned or solely propagated via word of mouth. That being said, after over 15 years of experience in gene therapy, it has become clear to me that a significant amount of crucial knowledge necessary to design and execute a successful gene therapy experiment often fails to be disseminated in a normal format (i.e., via scientific manuscripts). Of course, these benefits of gene therapy also translate to the use of gene therapy for the delivery of therapeutic genes in preclinical models of neurological disorders. For example, using this precision of gene therapy to model neurodegenerative disorders enables researchers to overcome any developmental compensations that may occur with germ line manipulations, to create lesions that are restricted to one hemisphere or specific circuits, and to easily titrate the genetic material of interest, among other benefits. One reason for this is the inherent ability of gene therapy to control genetic expression in both a spatial and temporal manner. Gene therapy is a valuable tool that is being increasingly utilized to model neurodegenerative disorders. Not only is gene therapy a useful utility in treating and creating preclinical models, but this technology has also demonstrated success in the clinic, in terms of both safety and efficacy. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (Ħ Pref ace Gene therapy of the nervous system, a technique once utilized by a few select laboratories, is now a commonplace research tool used around the world. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication.
#Phosphorylated asyn stereology free#
in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.
The use of general descriptive names, registered names, trademarks, service marks, etc. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Manfredsson Translational Science and Molecular Medicine Michigan State University, College of Human Science Grand Rapids, Michigan, USA ISSN ISSN (electronic) Methods in Molecular Biology ISBN ISBN (ebook) DOI / Library of Congress Control Number: Springer New York Heidelberg Dordrecht London Springer Science+Business Media New York 2016 This work is subject to copyright. Manfredsson Translational Science and Molecular Medicine, Michigan State University, College of Human Science, Grand Rapids, Michigan, USAĥ Editor Fredric P. Walker School of Life and Medical Sciences University of Hertfordshire Hat fi eld, Hertfordshire, AL10 9AB, UK For further volumes:Ĥ Gene Therapy for Neurological Disorders Methods and Protocols Edited by Fredric P.
#Phosphorylated asyn stereology series#
Manfredsson Editor Gene Therapy for Neurological Disorders Methods and ProtocolsĢ M ETHODS IN MOLECULAR BIOLOGY Series Editor John M. 1 Methods in Molecular Biology 1382 Fredric P.
0 kommentar(er)
